About Us
William R. Wiley Environmental Molecular Sciences Laboratory (EMSL)
This project involves the continued evolution and use of high-throughput proteomics capabilities developed to date at PNNL. Its primary aim is to provide effective proteomics measurements in support of DOE's Genomics:GtL program. The continuation of this project aims to address the rapidly growing demand for proteomics measurements (e.g. from BER-supported "Users" and collaborators, and specifically including the companion "Applications" project that serves as a prime test bed for large-scale applications).
While the primary aim is to provide proteome measurement production, this project also involves the refinement, advancement, and evaluation of technology advances in proteomics that will further augment data quality and throughput, and thus contribute to meeting longer-term GtL program research objectives.
Technology and instrumentation developments at PNNL have resulted in a facility continually advancing capabilities for proteome measurements. We have successfully demonstrated the utility of proteomics analyses to facilitate hypothesis driven research in various microbial systems by providing data that, for example, 1) improved the annotation of the Shewanella oneidensis MR-1 genome, 2) determined the distribution of proteins among different sub-cellular compartments in Rhodobacter sphaeroides, and 3) measured relative protein abundance changes in microbes between one growth state to another.
We are housed in the William R. Wiley Environmental Molecular Sciences Laboratory (EMSL), and the proteomics resource leverages and extends the substantial capabilities of the EMSL. These currently include a collaboratory infrastructure, unique mass spectrometric instrumentation, and a powerful suite of proteomic data management and analysis tools.
EMSL is a DOE user facility located on the campus of PNNL, a multi-disciplinary laboratory where scientists and engineers resolve critical challenges in energy, the environment, and national security.