This project involves the continued evolution and use of high throughput proteomics capabilities and facility developed to date at PNNL, and that has its primary aim to provide effective proteomics measurements in support of DOE's Genome GtL program. The continuation of this project aims to address the rapidly growing demand for proteomics measurements (e.g. from BER supported "Users" and collaborators, and specifically including the companion "Applications" project that serves as a prime test bed for large scale applications).
While the primary aim is to provide proteome measurement "production", an aspect of this project involves the refinement, advancement, and evaluation of technology advances in proteomics that will further augment data quality and throughput, and thus contribute to meeting longer-term GtL program research objectives.
Technology and instrumentation developments at PNNL have resulted in a facility continually advancing capabilities for proteome measurements. We have successfully demonstrated the utility of proteomics analyses to facilitate hypothesis driven research in various microbial systems by providing data that, for example, 1) improved the annotation of the Shewanella oneidensis MR-1 genome, 2) determined the distribution of proteins among different sub-cellular compartments in Rhodobacter sphaeroides, and 3) measured relative protein abundance changes in microbes between one growth state to another.
We are housed in the William R. Wiley Environmental Molecular Sciences Laboratory (EMSL), the Resource leverages and extends the substantial capabilities of the EMSL that presently include a collaboratory infrastructure, unique mass spectrometric instrumentation, and a powerful suite of proteomic data management and analysis tools.
The EMSL is a U.S. Department of Energy user facility located on the campus of Pacific Northwest National Laboratory (PNNL). PNNL is a multi-disciplinary laboratory where scientists and engineers resolve critical challenges in energy, the environment, and national security.
